Somatic Embryogenesis in Clerodendrum phlomidis L
نویسندگان
چکیده
Clerodendrum pholomidis is an important medicinal plant of Lamiaceae. Leaves, internode and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of 2, 4-dichlorophenoxy acetic acid (2, 4-D), kinetin (kn) and indole-3 butyric acid (IBA). Leaf explants formed clusters of globular somatic embryos directly from epidermal cells within 12-15d when cultured on Murashige and Skoog (MS) medium supplemented with 2.00 mg/l 2,4-D, 0.50 mg/l Kn and 0.025 mg/l IBA. Somatic embryo germination successfully achieved on plant growth regulator (PGR) free 1⁄2 MS medium.
منابع مشابه
Evaluation of Anti-inflammatory Activity of Root Bark of Clerodendrum Phlomidis in Experimental Models of Inflammation
The study was carried out to evaluate anti-inflammatory activity of aqueous extract of root bark of Clerodendrum phlomidis (CP) in models of acute and chronic inflammation in rats. Anti-inflammatory activity of CP was evaluated in models of acute inflammation viz. carrageenan induced rat paw oedema and acetic acid induced peritonitis in mice. The anti-inflammatory activity against chronic infla...
متن کاملEvaluation of fractions and 5,7-dihydroxy- 4,6-dimethoxy- flavone from Clerodendrum phlomidis Linn. F. against Helicoverpa armigera Hub
Twelve fractions from chloroform extract of Clerodendrum phlomidis and 5,7-dihydroxy4,6-dimethoxy-flavone (pectolinaringenin) were evaluated against Helicoverpa armigera. Maximum antifeedant (89.41%), larvicidal (83.77%) and ovicidal (69.25%) activities were observed in fraction 5. The least LC50 value for antifeedant (178.09 ppm) and larvicidal (198.23 ppm) were observed in fraction 5. No adu...
متن کاملInduction of somatic embryogenesis in absence of exogenous auxin in cucumber (Cucumis sativus L.)
Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis. Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...
متن کاملBioefficacy of Pectolinaringenin from Clerodendrum phlomidis Linn. F. against Anopheles stephensi and Bhendi Fruit Borer, Earias vittella fab
Larvicidal activity of pectolinaringenin from Clerodendrum phlomidis was evaluated against Anopheles stephensi and antifeedant, larvicidal and growth inhibitory activities were evaluated against Earias vittella. Pectolinaringenin exhibited larvicidal activity of 100 and 98.24% against 2 nd and 4 th instar larvae of Anopheles stephensi at 5ppm concentration. It exhibited LC50 values of 0.35 and ...
متن کاملSOMATIC EMBRYOGENESIS AND REGENERATION OF PLANTLET IN SAFFRON, CROCUS SATIVUS L.
Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...
متن کامل